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What is Grams Stain

Technological Innovation Driving Sustainable Entrepreneurial Growth in Developing Nations
This is a stain developed by one scientist called Christian Gram that is used to stain bacteria after they have been isolated from their culture.
Published in Chapter:
Commonly Used Public Facility Devices as Potential Sources of Infections
Maxwell Hubert Antwi (Koforidua Technical University, Ghana), Francisca Serwaa Adomako (Garden City University College, Ghana), Samuel Adomako Ocrah (Garden City University College, Ghana), Dorcas Serwaa (University of Melbourne, Australia), Aaron Siaw Kwakye (Koforidua Technical University, Ghana), and Isaac Darban (Koforidua Technical University, Ghana)
DOI: 10.4018/978-1-6684-9843-9.ch009
Abstract
Surfaces and devices in public institutions are likely to be contaminated with various microorganisms as people congregate there for various reasons. Swab samples from devices like ATMs, seats, teller counters, door handles, pens, writing desks, toilet flush handles, and tap heads were obtained from banks and churches. 60 samples in all were cultured and isolates were identified using Gram stain reaction, morphological, and biochemical characteristics. Results indicated a 100% microbial contamination on all surfaces with Staphylococcus aureus having the highest frequency, 35(31.5%). Bacilli spp had the next highest frequency, 23(20.7%). Klebsiella spp 13 (11.7%), Salmonella spp 13(11.7%), Enterobacter spp 13(11.7%), Serratia spp 6(5.4%), Citrobacter spp 4(3.6%), Proteus 3(2.8%) and Streptococcus 1(0.90%) followed in that order. Commonly used public facilities' devices could serve as potential sources of infections due to their microbial contamination. It is highly recommended amidst this pandemic to have frequent proper hand hygiene to avoid unknowing contamination.
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