CFPS, or in vitro protein synthesis, is the production of proteins using biological machinery in a cell-free system, i.e., without the use of living cells.
Published in Chapter:
From Cells to Cell-Free Platforms: A Comprehensive Overview
Mohammad Uzair (International Islamic University, Islamabad, Pakistan),
Hammad Qaiser (International Islamic University, Islamabad, Pakistan), Muhammad Asad Ghuffran (International Islamic University, Islamabad, Pakistan), and Asif Mir (International Islamic University, Islamabad, Pakistan)
Copyright: © 2023
|Pages: 21
DOI: 10.4018/978-1-6684-6577-6.ch001
Abstract
Cell-free systems are powerful tools in biotechnology, allowing researchers to study and manipulate biological processes outside of intact cells. This chapter provides a comprehensive introduction to cell-free systems, covering their principles, historical background, preparation methods, and various approaches used in research and applications. It emphasizes the advantages of cell-free systems, their historical development, and the different methods for preparing cell extracts or purified components. The chapter explores approaches like extracts-based cell-free systems and the PURE system, discussing their methodologies and applications. It also highlights the key components and factors influencing the efficiency of cell-free systems. Therefore, this chapter serves as a foundation for understanding the potential of cell-free systems in biotechnology research.