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What is Flow Cytometry

Applications of Image Processing and Soft Computing Systems in Agriculture
Is a technique performed by means of an apparatus called a cytometry whose objective is to analyze particles and/or cells suspended in a liquid medium and submitted to a flow, which simulates the passage of blood from a blood vessel. In this way, it is possible to perform the quantification, analysis, and classification of cells and/or particles.
Published in Chapter:
WT-MO Algorithm: Automated Hematological Software Based on the Watershed Transform for Blood Cell Count
Ana Carolina Borges Monteiro (Universidade Estadual de Campinas, Brazil), Yuzo Iano (Universidade Estadual de Campinas, Brazil), Reinaldo Padilha França (Universidade Estadual de Campinas, Brazil), and Navid Razmjooy (Independent Researcher, Belgium)
DOI: 10.4018/978-1-5225-8027-0.ch002
Abstract
Visual examination of blood smears is an essential tool for analysis, prevention, and remediation of several types of maladies. The interest of computer-aided decision has been acknowledged in many medicinal instances (e.g., automatic ways and means are being explored to spot, classify, and measure visual items in hematological cytology [HC]). This chapter proposes an entirely automated blood smear diagnosis system for hemograms, which can lessen the time spent to scrutinize a slide. The present framework relies on morphological operations (MOs) and soft segmentation by means of the watershed transform (WT). Experiments demonstrate the method efficacy to count white blood cells (WBCs) and red blood cells (RBCs). Some considerations about implementations, design advice and possible variants, as well as improvements are discussed. The future of automated medical analysis is contemplated.
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