The production of valuable compounds, such as pharmaceuticals, biofuels, and industrial enzymes, using living organisms or cell-free systems.
Published in Chapter:
Cell-Free Synthetic Biology: The Novel Approach Towards the Biotechnological Applications
Hammad Qaiser (International Islamic University, Islamabad, Pakistan),
Mohammad Uzair (International Islamic University, Islamabad, Pakistan), Abida Arshad (Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan), Jabar Zaman Khan Khattak (International Islamic University, Islamabad, Pakistan), and
Shahid Bashir (King Fahad Specialist Hospital, Dammam, Saudi Arabia)
Copyright: © 2023
|Pages: 20
DOI: 10.4018/978-1-6684-6577-6.ch002
Abstract
Innovations in sustainable biomanufacturing are made possible by cell-free synthetic biology advances. Cell-free workflows frequently combine purified enzymes with cell extracts for cell-free protein synthesis processes to create and analyze biosynthetic pathways and synthesize therapeutics. The development of cell-free platforms, high-yielding, cell-free gene expression systems, and multiplexed strategies for quickly evaluating biological design have all been made possible by recent technological advances in cell-free platforms, such as microfluidics, machine learning, CRISPR-mediated genome editing, etc. Developments in cell-free platforms satisfy many of the requirements of preparatory biochemical research, clinical purposes, diagnostic devices, industrial application, biosensors, etc. that offer exciting opportunities to transform synthetic biology and biotechnology. Therefore, this chapter aims to elaborate on the rapidly advancing field of cell-free platforms.